cDNA Microarray Screening for Taste-bud-specific Genes

cDNA microarray screening for taste-bud-specific genes.

Information about taste signaling molecules is still insufficient to understand the mechanisms of taste signal transductions, while novel taste receptors have been found recently. There are two strategies to discover novel taste signaling related genes; one strategy is homology based cloning and another strategy is global gene screening. Homology based cloning has been mainly used to find out t...

cDNA Microarray Data Normalization

Screening for candidate genes related to breast cancer with cDNA microarray analysis

OBJECTIVE The aim of this study was to reveal the exact changes during the occurrence of breast cancer to explore significant new and promising genes or factors related to this disease. METHODS We compared the gene expression profiles of breast cancer tissues with its uninvolved normal breast tissues as controls using the cDNA microarray analysis in seven breast cancer patients. Further, one ...

Taste bud regeneration and the search for taste progenitor cells.

While the taste periphery has been studied for over a century, we are only beginning to understand how this important sensory system is maintained throughout adult life. With the advent of molecular genetics in rodent models, and the upswing in translational approaches that impact human patients, we expect the field will make significant advances in the near future.

Screening and identification of peritoneal metastasis-related genes of gastric adenocarcinoma using a cDNA microarray.

With the aim of identifying peritoneal metastasis-related genes in gastric cancer, we performed a broad analysis of differential gene expression between the parental cell line GC9811 and its highly metastatic peritoneal counterpart, cell line GC9811-P. Two fluorescent cDNA probes, labeled with Cy3 and Cy5 dyes, were prepared from GC9811 and GC9811-P mRNA samples by the reverse transcriptio...